动物实验,心房钠尿肽,黑色素瘤肺转移 z-bo 2020-09-14 400

　　Objective: To investigate the effect of atrial natriuretic peptide (ANP) on melanoma lung metastasis.

　　Methods: The melanoma B16F10 cell line cultured in vitro was injected into atrial natriuretic peptide knockout mice (ANP-/-mice) and C57BL/6J mice through the tail vein to establish a melanoma lung metastasis model. On the 21st day later, count the number of nodules transferred to the surface of the lung, and after embedding and fixing the lung tissue, sectioning, and staining with HE, the lung metastasis nodules were identified and counted.

　　Results: The number of metastatic melanoma nodules on the lung surface of AP knockout mice was significantly less than that of C57BL/6J mice, and the difference was significant. The number of lung micrometastasis nodules in AP knockout mice was also significantly less than that of C57BL/6J mice. , The difference is significant.

　　Conclusion: ANP knockout significantly inhibited the lung metastasis of melanoma.