动物实验,miRNA-144 基因,肠道菌群 z-bo 2020-09-14 379

　　Objective To analyze the anti-tumor effect of microRNA-144 (miRNA-144) gene overexpression on liver cancer mice and its influence on the intestinal flora.

　　Methods To establish transplanted ascites liver cancer in mice. Observe the effects of miRNA-144 gene overexpression on the general condition of liver cancer mice, histopathological morphology of liver and intestine, the abundance and diversity of intestinal flora, and the intestinal barrier function. Real-time fluorescence quantitative polymerase chain reaction and Western blot were used to determine the Occludin, ZO-1, miRNA-144 mRNA and Occludin, ZO-1 protein.

　　Results The overexpression of miRNA-144 can improve the general physical condition of liver cancer mice, inhibit the development of liver cancer, improve the intestinal microecological environment and intestinal mucosal barrier function, and up-regulate the colon tissue Occludin, ZO-1, miRNA-144 mRNA and Occludin, Expression of ZO-1 protein.

　　Conclusion The overexpression of miRNA-144 can inhibit the development of primary liver cancer and improve the intestinal microecological environment and intestinal mucosal function of mice. The mechanism may be related to the up-regulation of Occludin and ZO-1 mRNA and protein expression.