How much do you know about rat breeding for animal modeling?
动物实验,实验用鼠
z-bo
2020-07-22
725
In the 17th century, mice began to be used in animal modeling. Today, the mouse has become the most widely used and detailed experimental animal. However, since mice have no sweat glands and prefer to live in a dark environment that is less adapted to the environment, you should always pay attention to details to avoid sick mice.
One. Warm reminder of the reproduction of "Xiao Bai"
As an albino mouse, it has its own concerns because it uses the white fur that other mice envy. Albinism is caused by the lack of tyrosinase (TYR) in animals with albinism. Albinism is the main enzyme for melanin production. Animals with albinism lack tyrosinase and cannot synthesize melanin. Albino animals are the product of a pair of recessive genes homozygous. Most of these irises are red and usually have other adverse effects on themselves, such as photophobia, nystagmus and skin cancer. Therefore, "Xiaobai" is most afraid of strong light, but in the daily breeding process, please do not put "Xiaobai" on the cage to match the lighting. This operation makes "Little White" eyes easier to appear. Blind people and breastfeeding mothers are more prone to neurological diseases and may eat offspring.
二. Nude mouse breeding techniques. In recent years, nude mice have played an active role in modern immunology and oncology research. Their appearance is very fragile. Due to the lack of thymus, T lymphocytes and low resistance, the reproduction conditions of nude mice are very strict, and a suitable barrier environment is essential for nude mice. Under breeding conditions, in addition to autoclaving or disinfecting the corresponding materials, more attention should be paid to the selection of garbage to ensure that the temperature and humidity are sufficient to ensure the survival of nude mice.
In daily feeding, dust often directly touches the skin of the mouse, so dusty dust should not be selected. At the same time, we do not recommend choosing solid materials to cultivate nude mice. In the daily material preparation process, it is necessary to observe whether the corncob bedding material after high pressure is agglomerated. We also do not recommend providing sturdy hard bedding for nude mice.
Of course, you should also pay attention to their food and drinking water, but if you are using a water bottle, you should change it frequently to avoid oral discomfort.
three. Pregnancy mouse reproduction alert
In the daily reproduction process, the more terrifying problem may be the discovery that some female mice feed on babies due to various factors. There are many reasons for the phenomenon of eating babies, including the reduction of births due to the strain of pregnant mice, poor fetal development and poor feeding during the production process. However, you can avoid some eating habits:
1. Noise. Pregnant mice should be very careful during delivery. If stimulated by noise, it can cause electric shock. During the daily lactation process, the pregnant mice should be observed regularly, and sufficient feed and drinking water should be supplemented before delivery to ensure that they will not interfere with the mouse's mother during delivery. If it is necessary to operate, keep it gentle.
2, nutritional factors. Pregnant mice have stricter nutritional requirements for protein and vitamins. Literature studies have shown that feeding dietary supplements during pregnancy can increase the physique and fertility of pregnant and lactating female mice. Therefore, daily observation needs to pay attention to the hair and mental state of mice to determine their healthy nutritional status.
3. Stocking density. In the daily breeding process, given the number of pregnant rats and puppies, the breeding density needs to be strictly controlled. In order to control the density of livestock and ensure the health of animals, Jicui Yaokang eliminated the 1:3 multiplication.
Practice, for different strains, they have unique properties under almost the same conditions. For example, diabetes model mice need to be improved with the characteristics of "more than three and one less". The frequency of changing cages ensures their living environment. For example, in daily feeding, mice are extremely small compared to their siblings. If it is not a pathological factor, it can be stored separately. The feed crushes the feed and grows slowly. Of course, don't forget that the environment is rich. We provide it with mouse toys, keep neighbors and avoid being alone.
Carefully protect laboratory animals not only to follow the principles of protecting animal welfare, but also to make laboratory data more accurate.
The self-development of sterile consciousness, daily observation, accumulated experience, we are still paying attention to patience and the pursuit of details.
