动物实验,体外受精 z-bo 2020-09-14 413

　　OBJECTIVE: To study the effects of two type I interferon receptor deficiencies on in vitro fertilization in mice and optimize the conditions for in vitro fertilization.

　　Method: How to perform in vitro fertilization and embryos in two type I interferon receptor-deficient mice (IFN-αR-/-, IFN-α/βR-/-) and background wild-type mice (C57BL/6) For transplantation, record five superovulated mice in each group, three replicates and related data to analyze whether the lack of interferon receptors affects the in vitro fertilization of mice. Two type I interferon receptor-deficient mouse partners and C57BL/6 mouse partners were subjected to in vitro fertilization hybridization experiments. Each group of 5 mice was performed three times. Both male and female partners had type I interferon receptors. Body defects. The effect of fertilization on offspring was studied in vitro. At the same time, we will consider technical methods to optimize in vitro fertilization conditions and increase fertility. 5 mice per group, repeat 3 times.

　　Result: The average in vitro fertilization rate of two type I interferon receptor-deficient mice is lower than the background strain C57BL/6 mice, and the difference between the two groups is significant (P\u003c0.05). The in vitro fertilization rate of mice lacking interferon α and β receptors was higher than that of mice lacking interferon α and β receptors, and the difference between the two groups was significant (P\u003c0.05). The in vitro fertilization rate of sperm from two type I interferon receptor-deficient mice and C57BL/6 mouse oocytes was higher than that of oocytes and C57BL/6 mouse sperm, the difference between the two groups Significantly. (P\u003c0.05). Extending the fertilization time of sperm to 1 hour, or adding 1 mmol/L reduced glutathione (GSH) to fertilization and fertilizer can increase the in vitro fertilization rate, and it varies between the corresponding groups. This will make sense (P\u003c0.05).

　　Conclusion: The lack of type I interferon receptors will reduce the in vitro fertilization ability of corresponding strains of mice, and the impact on egg cells is more important than the impact on sperm. The in vitro fertilization rate can be improved by appropriately extending the fertilization time of sperm or changing the fertility and fertilizer composition.