Early mouse embryonic stem cell models are more accurate
动物实验,小鼠胚胎
z-bo
2022-09-02
604
Scientists at the Hub Hubrecht Institute (KNAW) and Cambridge University (UK) have successfully produced complex embryo-like structures from mouse embryonic stem cells. These structures, called gastric colloids, allow the ovaries to grow for the first time, and then to the spine and muscles of the embryo. This is the first time that we have successfully produced a high-level embryo-like structure, representing this stage of embryonic development after implantation in the uterus. This model system allows us to study the late stages of these embryos in petri dishes. Their research results were published in the journal Nature on February 19. During embryogenesis, the fertilized egg develops into a complete organism. However, the process leading to the development of mammalian embryos remains unclear. For example, do mammals need to develop a heart or the number of vertebrae that make up the spine, or which side of the body? Problems may arise during these processes. How did this happen? Can you prevent it? Which compounds are good for embryo development and which are bad? Research to solve these problems usually requires mouse embryos. However, these embryos develop in the uterus and are difficult to obtain in large quantities.
Somites
In 2014, these scientists took the lead to grow embryoid-like structures from mouse stem cells. At present, they have further improved the culture conditions to increase the similarity between the embryoid body and the embryo, and the embryoid body grows in the bean node. The somites are "small pieces" of small tissue on the back of the embryo, which then form the vertebrae and skeletal muscles. Therefore, compared with previous models, current stomach-like organisms are more complex in mouse embryos and more like mice. Suzanne Vandenbrink of the Husbrecht Institute: "This is the first time we have been able to generate such a complex embryo-like structure to summarize the stages of embryonic development that usually occur after implantation in the uterus." Vincent van Ba of the Hubrecht Institute Vincent van Batenburg added: "
Gastrobud has made a detailed comparison of embryoid body and embryo by examining the structure of embryoid body and embryo. We are also studying the following cell types and tissues: the position of the tail shaft, to determine when the cell type is determined by the combination of active genes, Which positions are active in the embryo and embryoid body. You can pass
to study these cell types. most advanced
Therefore, scientists are using the most advanced technologies, such as single cell sequencing and tomo sequencing. , Can be used to measure the total set of active genes in each single cell, but does not provide information about the location of these cells. Chromatography, then the embryo or embryoid body is cut into thin slices. A cutting method in which thin slices are cut from beginning to end, and then
Proper model system
single cell and tomography sequence to measure the gene activity of each part combined, scientists can accurately measure the cell types present in the steroid and the position of the steroid and embryo on the head and tail axis. , Which makes them a very suitable model system for studying embryonic development. Anna Allemanie: "However, the important difference between embryo and gastrin is the reproductive glue. There is no brain or placenta, so these structures are not feasible.
