【Animal Experiment】-The effects of four organic solvents on zebrafish eye development
动物实验,有机溶剂
z-bo
2020-07-24
711
Objective To evaluate the effects of ethanol, isopropanol, acetone and dimethyl sulfoxide on zebrafish embryonic development, especially eye development, and to provide references for its application in the study of zebrafish eye disease models.
Method Use 0.5% ethanol, 1% isopropanol, 1.5% acetone, and 2.5% dimethyl sulfoxide to expose zebrafish embryos from 12 hpf to 72 hpf to observe their viability, teratogenicity and eye development. Results The survival rates of the control group and the ethanol, isopropanol, acetone and DMSO exposure groups were 96%, 93%, 90%, 90% and 86%, respectively. The zebrafish fry in the control group had normal morphology, while the group exposed to organic solvents showed varying degrees of morphological changes, such as pericardial edema, hemorrhage, lack of egg yolk and body curvature. Among them, DMSO has the most serious teratogenic effect, and ethanol has the weakest teratogenic effect. As a result of analysis of variance, there was no significant difference in the eye length/body length ratio of juvenile zebrafish between the organic solvent-exposed group and the control group. This ratio is significantly lower than other groups. In addition, compared with the control group, the retinal stratification of the four organic solvent exposure groups was normal. The signals of pyramidal cells, rod cells and retinal neuron cells in the ethanol, isopropanol, and acetone exposure groups were normal, but the signals of the DMSO exposure group were different. Cell signal is weak.
Conclusion Therefore, when using zebrafish as a model animal for eye disease-related research, please carefully select organic solvents as the dissolution carrier of drugs or other chemical test products, and conduct preliminary experiments to determine the optimal concentration.
