动物造模,冠心病 z-bo 2020-09-25 311

　　Objective: To use liquid chromatography mass analysis (LC-MS) technology and asymmetric metabolomics technology to study the characteristic metabolites of the flora of patients with coronary heart disease.

　　Method: 28 sterile female C57BL/6J mice were divided into control group (CON) and model group (CAD), and 6 and 10 weeks after transplantation, fresh feces of healthy volunteers and patients with coronary heart disease. Inoculate with cloudy liquid. Seven animals in the Henle group collected plasma, used LC-MS technology to study mouse plasma metabolites, and used PCA and PLS-DA statistical techniques to correlate the characteristic metabolites.

　　Result: It was finally confirmed by a standard product containing 30 different metabolites at two time points. Among them, the model group L-carnitine, phenylpyruvate, 1-naphthol and 2-naphthol significantly increased. In the 6th and 10th week of modeling, the metabolic pathways of bile acid, glycine and threonine were all down-regulated.