动物造模,深Ⅱ度烫伤 z-bo 2020-09-25 389

　　Objective: To establish a pig model of second degree burn and observe the histopathological changes of skin repair at different times.

　　Method: Use a constant temperature burn device to perform deep two-degree burns on the skin of mini pigs. The macroscopic morphological changes were observed on the first 1, 3, 7, 4, 21, and 28 days of wound healing to measure the wound healing rate. According to the law of skin tissue healing and experimental operability 1. Use HE, Masom staining, and immunohistochemical CD31 staining to sample on the 14th and 28th days, and perform dynamic skin repair at different time points to observe various pathological changes.

　　Result: According to the measurement of wound healing rate, the burn wounds of size pigs heal faster than sows. It has been confirmed that the skin tissue after burns has accumulated damage in the deep layer of the dermis through various staining methods, and has reached the standard for deep second degree burns. On the 14th and 28th days of the subsequent selection, the skin tissue was repaired to varying degrees after injury. 28 days after the deep second degree burn, the skin of the mini-pig has basically healed, but the skin tissue is not yet fully mature. Except for the relative maturity of the various layers of the epidermis, the dermis has not yet fully formed scar tissue.

　　Conclusion: Compared with male pigs, male pigs heal faster than female pigs.