动物造模,猪圆环病毒2型 z-bo 2020-09-25 304

　　Purpose: The possibility of porcine circovirus type 2 (PCV2) infecting human cells has been controversial. This study uses transcriptome sequencing (RNAsequencing, RNA-seq) technology to analyze the differentially expressed genes, related signal pathways and biological functions of PCV2 after infection of human cells. The research provides a reference.

　　Method: Infect HeLa cells with PCV2, and perform RNA sequence analysis to screen for genes with differential expression related to antiviral response (DEG), and further verify by real-time quantitative PCR.

　　Result: In HeLa cells infected with PCV2, a total of 15,402 UniGenes were annotated, including 387 DEGs, including 267 up-regulated genes and 120 down-regulated genes. The KEGG pathway analysis of GO enrichment and biological process DEG found that the enriched genes are involved in stress response (97/291DEG) and immune system processes (80/291DEG). These are the two most important pathway genes that are NOD-like receptors. body. Differential expression of genes related to somatic signal transduction pathway (21/170DEGs) and herpes simplex virus infection pathway (22/170DEGs).

　　Conclusion: When PCV2 infects HeLa cells, the expression profile of the cells changes. DEG and the obtained functional annotation information are very useful for understanding its antiviral mechanism, as well as understanding the immune response and antiviral effects of human cells infected with PCV2.