动物造模,哺乳期小鼠 z-bo 2020-09-25 465

　　Objective: To explore the artificial feeding method of lactating mice, to lay the foundation for further research on the nutritional requirements and growth of mice.

　　Method: Raise 10-12 days old SPF incoherent BALB/c pregnant mice by conventional methods until spontaneous. 37 3-day-old newborn mice were randomly divided into artificial feeding group and breast-feeding group. Breastfeeding on the 14th day. As a result of 3 to 10 days after birth, the survival rate of mice in the artificial feeding group was 100%, 92.9% at 11 days, 78.6% at 12 days, 50% at 13 days, and 35.7% at 14 days. A total of 5 mice in the artificial feeding group successfully fed 14-day-old mice. However, at 10 days of age, the weight of mice in the artificial feeding group was significantly smaller than that in the breastfeeding group (3.02±0.67 vs 9.00±0.70, P\u003c0.01).

　　Conclusion: In this experiment, we used our homemade pacifiers and artificial mouse milk to achieve a certain degree of artificial feeding of mice, but it is necessary to consider ways to increase the survival rate of mice during artificial feeding.