动物造模,DNA标记 z-bo 2020-09-25 368

　　Objective: To conduct genetic testing of various production and breeding populations of Guangxi Zhuang macaques, analyze the genetic background of different breeding populations of Guangxi Zhuang macaques, and conduct genetic quality testing methods and populations of Guangxi Zhuang macaques.

　　Method: Provides basic information about the establishment of a resource bank. It uses 20 microsatellite loci and capillary electrophoresis technology to detect the spread of the three different crab test production groups in Guangxi Zhuang Autonomous Region and each group. We calculated the genetic variation parameters within and between groups.

　　Results: 237 alleles were detected, the number of alleles observed (Na) was 5-19, the average was 11.85, the average expected heterozygosity (He) was 0.85, and the average polymorphic information (PIC) was 0.817 . 158, 158, and 173 alleles were detected in three different production groups (F, N, and W). The average expected heterozygosity (He) was 0.8371, 0.8318, and 0.8642, respectively, and the average polymorphic information (PIC) was respectively It is 0.7692, 0.7653 and 0.8001, and has high genetic polymorphism in three different production groups. Convenient balance test of Weinberg's three production groups. Most sites are hardware balanced. The average intimacy coefficient Fis of this group is 0.0235, the average intimacy coefficient Fit is 0.0628, the average gene differentiation coefficient Fs is 0.0402, and the average gene flow Nm is 5.9616. The three production groups are basically in a random mating state. There is almost no genetic differentiation among populations. The genetic distance between the three production groups is 0.2556-0.3223, and the genetic similarity is 0.7245-0.7745. Cluster analysis shows that the populations of F and N are first grouped together, and then matched with the three breeding monkey farms. Population. Associate referrals with historical characteristics.

　　Conclusion: This study effectively analyzed the genetic polymorphisms of the Guangdong crab monkeys and the genetic relationship between populations. The selected 20 microsatellite loci can be used for genetic quality testing of cynomolgus monkeys.