动物造模,卵细胞 z-bo 2020-09-25 357

　　Purpose: Collect immature follicular eggs from nonovulated ovaries after superovulation, and mature oocytes in vitro to improve fertility, thereby increasing the utilization rate of mice. As a remedy for failure of regular ovulation.

　　Method: In biologically purified mice, the non-ovulated ovaries were harvested after PMSG and HCG infusion, the follicles were lysed under a solid-state microscope, and mature droplets were selected by selecting the mountain egg stromal cell complex (COC). Placed in vitro and matured. At the same time, normal superovulation after PMSG and HCG infusion, in vitro maturation of immature eggs after PMSG and HCG infusion, and in vitro maturation of suspected mature eggs after PMSG and HCG infusion were used as controls. After in vitro fertilization and in vitro embryo development, the two-cell embryo is transplanted into the recipient's oviduct and grows into a fertilizer mature individual. Results After PMSG and HCG were injected into the non-ovulatory group (group A), the in vitro maturation rate of oocytes was 87.0%±3.2%, and the in vitro maturation rate of the two cells was 55.1%±12.3%. The transplantation rate was 23.1%, and 41 2-cell embryos were transplanted. Two pups gave birth to 5 puppies, and the number of litters was 12.2%. Only PMSG was injected, and the immature egg group was collected 48 hours later (group B). The in vitro maturation rate is 83.9%±3.9%, the ratio of two cells is 51.8%±9.3%, and the ratio of scutellum is 38.5%±13.9%. In the PMSG and HCG normal high ovulation group (group C), the incidence of enterotoxins was 78.9%±0.6%, and the incidence of scutellum vesicles was 78.0%±3.8%. Inject PMSG and HCG into non-ovulating ovaries, suspected naked eggs and mature eggs (group D), in vitro maturation and culture for 0 hours, 6 hours, 16-18 hours, maturation rate, ratio of 2 cells, compare the other three groups of cysts proportion. Low and very significant difference. Compared with the normal control group C, the ratio of the two cells in group A and group B is very different, and the ratio of scutellum is also very different.

　　Conclusion: In vitro maturation of oocytes (IVM) can be used to treat excessive ovulation failure in mouse biological purification, and can improve the utilization of eggs in rare mouse strains.