肠道菌群,动物造模 z-bo 2020-09-25 341

　　Objective To explore the differences in intestinal flora between guide dogs and culled dogs, and analyze the characteristic differences between the two.

　　Methods The fresh feces samples of 16 guide dogs and 10 eliminated dogs were collected and divided into Labrador male guide dogs (GD-ML) and eliminated dogs (ED-ML) according to dog breed and sex, and Labrador Female guide dogs (GD-FL) and elimination dogs (ED-FL), golden retriever male guide dogs (GD-MG) and elimination dogs (ED-MG), and 9 guide dogs and 4 elimination dogs are randomly selected from them Dogs are divided into guide dog group (GD) and elimination dog group (ED), which are divided into 4 groups. Extract the total DNA of stool samples, use PCR-DGGE technology to obtain the intestinal flora map, and use relevant software and statistical methods to analyze the differences of each pair of pairs.

　　Results The cluster analysis results showed that GD and ED, GD-ML and ED-ML, GD-FL, ED-FL and GD-MG were all classified into one category, indicating the intestinal flora between guide dogs and eliminated dogs has a difference. The analysis results of the diversity, richness and evenness of the intestinal flora showed that there were differences in the diversity index, richness index and evenness index between every two paired groups, but the differences were not statistically significant. Sequencing analysis results of differential bands showed that: compared with ED, CD Megamonas funiformis YIT 11815 strains increased; compared with ED-ML, GD-ML Succinatimonas hippei YIT 12066, Lactobacillus vaginalis DSM 5837, Lactobacillus acidophilus NCFM, Faecalibacterium prausnitzii A2- 165 strains increased, Collinsella aerofaciens ATCC 25986 and Prevotella copri DSM 18205 strains decreased; compared with ED-FL., GD-FL Ruminococcus gnavus AGR2154, Fusobacterium russii ATCC 25533 strains decreased; compared with ED-MG, GD-MG Tropheryma whipplei Str. Twist strains increased.

　　Conclusion This study found that there are differences in the intestinal flora of guide dogs and eliminated dogs. Further research on the different flora in the future may help assist in the early screening of guide dogs.