动物造模 z-bo 2020-09-25 422

　　Purpose: Selection and breeding test are closely related to hip dysplasia and hope to become Labrador and Golden Retriever puppies with 3 SNPs of genomic DNA COL6A3 and FN1.

　　How to hire 8 Labrador Retrievers with hip dysplasia and 5 Golden Retrievers across the country. Healthy Labrador with imaging diagnosis provided by Dalian Blind Dog Training Base in China 23 Use two multi-headed dogs and seven The golden retriever was used as the experimental object. The genomic DNA sample of blood cells is extracted by collecting blood in the vein intravenously. Two SNP sites (CFA25: 51031100, 510040259) and the FNI gene of the COL6A3 gene were analyzed using the PCB/sequencing method. Determine the genotype of a SNP locus (CFA37: 25095511), and statistically analyze the correlation between the genotype of the SNP locus and bone marrow dystrophy.

　　Result: The SNP locus of COL6A3 (CFA25: 51031100, 51040259) and FNI (CFA37: 25095511) genotypes has no significant correlation with large-scale hip dysplasia (P\→ 0.05).

　　Conclusion: COL6A3 (CFA25: 5103100, 51040259) and FNI (CFA37: 25095511) SNP loci cannot be used as practical genetic diagnostic indicators for papillary dysplasia in Labrador and Golden Retriever dogs.