动物造模,肺炎模型 z-bo 2022-02-03 553

      (1) Replication method The CWL2029 strain of Chlamydia pneumoniae was cultured in HeLa299 cells, the infected cells were destroyed by ultrasound, and then the pathogen was purified by differential centrifugation and sterile sucrose diphosphate at a concentration of 5.3×100000000 IFU/ml. Resuspend in the buffer. Take 4-5 weeks old mice. First, the mice were gently anesthetized by inhaling ether to induce hyperventilation. Mice use a syringe with four and a half needles to draw 0.05 ml of inoculum into their nostrils (approximately 2.7 x 10000000 IFU). After intranasal inoculation of Chlamydia pneumoniae, their vitality decreased and their diet decreased within 2 days, and they usually returned to normal within 1 week.

　　(2) Characteristics of the model In a mouse model of chlamydia pneumonia established by the intranasal inhalation method, pneumonia characterized by patchy interstitial pneumonia occurred, mainly due to more severe inflammation. Most animals can recover naturally after a week.

　　 (3) The comparative drug Chlamydia pneumoniae has not been found for a long time, and its cause is still unknown. The common symptoms of patients are fever, cough, sore throat, bronchitis and pneumonia, mostly mild. Up to 50% of adults have antibodies against Chlamydia pneumonia, but most people do not experience symptoms of pneumonia. After intranasal inhalation of Chlamydia pneumonia, the main symptoms of pulmonary interstitial pneumonia were obvious congestion and ecchymosis, and the symptoms disappeared after two weeks. The early inflammation is dominated by the infiltration of neutrophils and the accumulation of foam cells, and the later stage of the disease begins to relieve. The mixed infiltration of neutrophils and lymphocytes is the main form, which gradually changes to lymphocyte infiltration.

　　 In addition, all model animals can produce higher titers of IgG antibodies. The mouse Chlamydia pneumonia model can be used to study the pathogenicity and immune mechanism of the disease, as well as drug screening and other research.