淋巴细胞,荧光RPA检测 z-bo 2020-09-25 379

　　Objective: To improve the surveillance and epidemiological investigation of wild and experimental wild and experimental primate monkey T lymphocyte trophic type 1 (simian T-cell lymphovirus type 1, STLV-1) infections. In addition, this research is a kind of Enzyme amplification (RPA) and fluorescence RPA methods for detecting STLV-1 in established recombinase polymerization reactions.

　　Method: Software, analyzed the gagpolyprotein gene sequence of STLV-1 isolated from different countries and regions, designed RPA primers and probes, and established RPV and fluorescent RPA methods. Test STLV-1 and determine the specificity of the method. It has been proven to be sensitive and stable. Compare with STLV-1

　　Other specific pathogens in monkeys confirmed that the established detection method has excellent specificity. Through the sensitivity test, it was confirmed that the established RPA and fluorescent RPA method had the same detection limit as PCR, and passed 30 copies; the detection of STLV-1 positive and negative nucleic acid samples can establish RPA and fluorescent RPA. It is confirmed that this method has the same stability and reliability as the PCR method. Conclusion The RPA and fluorescent RPA methods established in this study for the detection of STLV-1 have excellent specificity, sensitivity and reliability, and can be used for STLV-1 surveillance and epidemiological studies.