动物造模,超排卵,卵母细胞 z-bo 2020-09-25 384

　　Objective: To study the superovulation effect of C57BL/6J female mice and the effect of dehydroepiandrosterone (DHEA) on oocyte quality and its mechanism.

　　Method: 30 3-week-old C57BL/6J female mice were randomly divided into 3 groups: control group, low-dose treatment group (2 mg/kg? D-1) and high-dose treatment group (5 mg/kg). Divided? d-1). ). In vitro fertilization was performed with DHEA for 1 week, and stone cells were collected after excessive ovulation. RNA was extracted from oval cells for qRT-PCR to detect embryo quality Grem1, Has2, Ptgs1, Ptgs2, Vcan-related genes and apoptosis-related genes Caspase3 and Bcl2l10 mRNA levels.

　　Result: In vitro fertilization (IVF) showed that after excessive ovulation in female mice treated with DHEA, the proportion of atypical eggs in the experimental group decreased. Compared with the control group, the transcription levels of genes Has2, Ptgs1 and Vcan related to embryo quality in the experimental group's follicle cells were up-regulated, while the mRNA levels of Germ1 and Ptgs2 were down-regulated. Caspase3 mRNA level was down-regulated, and Bcl2l10 mRNA level was up-regulated.

　　Conclusion: After treatment with dehydroepiandrosterone (DHEA), the quality of oocytes can be improved by inhibiting ketone death of cumulus cells.