动物造模,慢性肝损伤周期性病变 z-bo 2020-09-25 378

　　Objective: To study the characteristics of liver damage in a mouse model of chronic liver disease induced by carbon tetroxide (CCL4), and to explore its application in medical research.

　　Method: Normal BALB/c mice were raised for 1 week and injected with 0.5% CCL4 solution (10μL/g, 1/3 day, 10 weeks). At 2, 4, 6, 8 and 10 weeks after injection, mouse serum was collected to detect ALT and AST, and liver was collected at the same time, and HE staining and Masson staining were used to observe the changes of liver injury in mice. Achieved the result. When CCL4 solution was injected for 2 weeks, the liver cell damage was mainly degenerative, with a large amount of inflammatory cell infiltration and a small amount of collagen deposition, and a slight increase in ALT and AST. In the 4th week, liver cell damage is mainly necrosis. (P\u003c0.01), the number of hepatocytes (P\u003c0.01) and inflammatory cell infiltration decreased (P\u003c0.01), collagen deposition evolved into linear collagen fiber deposition, ALT and AST significantly increased ( P\u003c0.01)); After 6 weeks, hepatocyte necrosis was significantly reduced (P\u003c0.01), and the hepatocyte count increased relatively (P\u003c0.01). , The infiltration of inflammatory cells is also reduced (P\u003c0.01), collagen fiber deposition is transformed into collagen deposition (P\u003c005), ALT and AST are basically normal, within 8 weeks, liver cell degeneration increased again, with a large number of inflammatory cells Infiltration (P\u003c0.01), ALT and AST increased slightly, within 10 weeks, liver cell damage and necrosis became the main reason again. (P\u003c0.01), the number of hepatocytes (P\u003c0.01) and inflammatory cell infiltration decreased (P\u003c0.05), collagen deposition evolved into linear collagen fiber deposition, ALT and AST have greatly increased. High (P\u003c0.01).

　　Conclusion: Low-dose CCL4 will induce chronic liver injury in mice, and the liver injury cycle will undergo obvious and regular changes. In the same cycle, the alternate sequence of "damage, necrosis, regeneration, repair" dominates the pathological changes of liver tissue. A corresponding "window period" should be selected to study the mechanism of liver damage or repair and evaluate its effectiveness.