动物造模,染色体畸变 z-bo 2020-09-25 322

　　Purpose: By administering cyclophosphamide (CP) and corticosteroids (COL) at different time points, and comparing different concentrations of different administration/collection time points, ICR mice were used to conduct in vivo chromosomal abnormalities experiments. The effect of chromosomal abnormalities on mouse bone marrow cells.

　　Method: A total of 36 male ICR mice, approximately 7-8 weeks old, were randomly divided into 18 hours after drug treatment and 24 hours after drug treatment, and then COL 4 mg/kg (4 hours before sampling, it was divided For intraperitoneal) and 10 mg weight. CP0 mg/kg, 10 mg/kg and 40 mg/kg were administered in the /kg (2 hours before sampling, intraperitoneal) administration group, with 0.9% sodium chloride injection as a vehicle control, 3 animals in each group. During the experiment, the body weight of the animals before and after the administration was recorded. The negative control group and the 10 mg/kg CP group were given twice every 24 hours with a single dose of 40 mg/kg CP. Samples were taken 18 or 24 hours after administration, and COL was injected intraperitoneally 2 or 4 hours before sampling. When collecting materials, collect all bilateral femurs of experimental animals, collect bone marrow cell suspension, prepare Ciemsa chromosome specimens, analyze the types of chromosomal abnormalities, and the average structural abnormal cell rate (ctg). And csg (excluding AC%) and the ratio of structurally abnormal cells in each group (including, for example, and esg, ACG%), multiple abnormal cell rate (excluding ctg and esg, MAC%), polyploid cell rate (PC%) ).

　　Result: The use of CP and COL in this study did not significantly affect the body weight of the animals, and the dose and frequency of administration are possible. Compared with each group of vehicle control group, AC%, ACG% and MAC% of all CP treatment groups were significantly increased (P\u003c0.01), and at higher doses, chromosome damage was more serious. The AC%, ACG% and MAC% of the CP18h-COL4h group were lower than those of the CP18h-COL2h group, with significant difference (P\u003c0.05), and high-dose COL (10 mg/kg) was added. This shows that it can cause chromosome damage. Most structural abnormalities in the vehicle control group are cracks, and chromosomal monomers and chromosomal breaks are the most important types of abnormalities caused by CP.

　　Conclusion: When performing chromosomal abnormality tests in ICR mice, it is ideal to obtain materials about 18 hours after CP administration and 4 hours after COL administration. This research not only accumulated background data by exploring experimental conditions, but also provided references for related researchers.