动物实验 z-bo 2020-09-26 341

　　Researchers at the University of Pittsburgh School of Medicine used porcine extracellular matrix (ECM) to induce stem cells into muscle cells through animal experiments. The study found that transplantation of these cells can regenerate muscles lost due to trauma or military injuries.

　　According to the latest research report of 5 patients, it can recover a large amount of muscle loss caused by trauma or military injury. After injury, skeletal muscle can regenerate, but it cannot regenerate under extreme conditions that destroy a large amount of skeletal muscle (this condition is called bulk muscle loss or VML). The treatment of VML is so limited that it is difficult for patients to perform daily activities. Stem cells have been shown to help regenerate damaged tissues. Most stem cell therapies follow the same pattern. Researchers isolate stem cells, grow them into cells of interest, and then inject them into patients. Stephen Badylak and Brian Sicari and colleagues developed another stem cell technology that uses pig extracellular matrix (ECM) to convert stem cells into muscle cells without adding stem cells. In the first proof

　　After the ECM method is effective for rodents with severely injured hind limbs, the researchers applied it to patients who lost 58-90% of their limb muscles. They tested the technology in 5 male patients who were at least 6 months away from the injury site, and the surgery and physical therapy of these patients were unsuccessful. Three of the patients were soldiers, two were injured by the explosion of an instant explosive device (IED), and the other was injured by sports. The other two patients were civilians who injured their leg muscles as a result of the ski accident.

　　Before the operation, all patients completed a 12-16 week physical therapy program for their functional defects. When these treatments stagnated, there was no sign of further improvement, and all patients received ECM stent implantation at this time. Researchers used surgical methods to remove scar tissue from these patients and added ECM materials. Then, each patient immediately resumed the preoperative physical therapy program and received physical therapy for 5-23 weeks. The indicator of treatment success is that the ability of these patients is at least improved compared with the ability of the largest patients to perform daily activities before surgery. 25% (raise one leg when finished walking, stand up from the chair, raise one leg to a sitting position, etc.). For every patient, their muscles will regenerate, and this regeneration will partially restore the appearance of normal muscles. After imaging the muscles treated by MRI and computed tomography, the researchers found that dense tissue formed at the transplant site 6 months after the operation. After transplantation, the matrix scaffold material decomposes and acts as a guide and return device to attract stem cells to the injury site. These stem cells then grow into muscle cells, thereby promoting the development of new healthy muscle tissue. The quality of life of all patients improved, but the researchers determined that 3 out of 5 patients improved their function by 25%. The author of the article said that post-treatment physical therapy is very important for the effectiveness of treatment. Physics may trigger the generation of signals that direct stem cells to become muscle cells instead of other specific types of cells. It is more effective to treat newly injured soldiers such as newly wounded soldiers. Buddylac said: "Able to treat patients immediately will benefit from this technology."