动物实验,神经脊细胞 z-bo 2020-09-26 400

　　Researchers at Harvard Medical University Massachusetts General Hospital led by Dr. Liao, Dr. Besty Chinicle and Dr. Wang Yaway, first cultivated the pluripotent characteristics of neurospinal cells isolated from zebrafish embryos. Neurospinal cells are a unique cell group induced by the lateral boundaries of the neural plate, and rely on these pluripotent and mobile cells during embryonic and spinal development. .. The developmental defects of the neural striae can cause a variety of deformities, including splits, palate and melanoma. Dr. Liao’s laboratory uses zebrafish as a vertebrate model to study the genetic roots of cranial and facial deformities related to neurostria. Zebrafish is used to study early development and has recently been developed as a model for studying disease. The lead author of the study, Dr. Ryo, Assistant Professor of Surgery at Massachusetts General Hospital and principal faculty member of the Harvard Stem Cell School, said: "For in vivo experiments in zebrafish, it is very important to develop in vitro culture of neurospinal cells and reproducible functional tests. We Provides a valuable supplementary method."

　　The team used the gene transfer model of the reporter gene SOX10 to amplify and isolate neurospinal cord cells and culture them in a suitable environment. It was found that the cultured neural spinal cord cells have the ability to express. Neural spinal markers, such as SOX10, sox9a, HNK1, P75, dlx2a, PAX3 transcription factors, and pluripotency markers, such as c-myc and KLF4, these cells can also differentiate into a variety of neurospinal systems, including nerve cells, Glial cells, smooth muscle cells, melanocytes and chondrocytes. The functional cell behavior analysis developed by the team can be used to evaluate the effect of the endogenous synthetic, effective and morphogenetic molecule retinoic acid on the behavior of neurospinal cells. This study shows that retinoic acid has a profound effect on the morphology and differentiation of neural c cells, especially in inhibiting differentiation and promoting cell migration. These data indicate that the neurospinal cell population is the target of retinoic acid, which means that retinoic acid plays a different and important role in the development of neurospinal cells. Dr Liao

　　Dr. Besti Chinicolu, a post-doctoral researcher in the laboratory and the first author of the study, said: "We hope that new neurospine system research can help us understand the developmental mechanism of neurospinal cells, and the application of cell-based high-throughput drug screening is useful. ."

　　Dr. Goodman, Chief Editor of Experimental Biomedicine, said: "Dr. Liao and his research team were the first to study the development of neurospinal cells. They provided an in vitro model of neural c-cell populations purified from zebrafish embryos. This Very valuable. I think it will be a tool."