动物实验,造血干细胞 z-bo 2020-09-26 443

　　Hematopoietic stem cells are a group of multipotent hematopoietic progenitor cells necessary to maintain normal physiological functions of the body, and can grow into various mature blood cells, such as red blood cells, bone marrow cells and lymphocytes. The hematopoietic process of zebrafish is divided into two stages: primary hematopoiesis and secondary hematopoiesis. Previous studies have shown that transcription of E. coli colipressorcor2 is involved in primary hematopoiesis, but its influence on secondary hematopoiesis, especially the production of hematopoietic stem cells, remains unclear. by

　　The blood and cardiovascular development research team led by researcher Liu Feng found that ncor2 is specifically expressed in the secondary hematopoietic regions of zebrafish-aortic gonadal-neutron kidney (AGM) and hematopoietic stem cells (HSC). done. Knockout or knockout of ncor2 can reduce the number of hematopoietic stem cells and T cells. Further studies have shown that Ncor2 and Hdac3 jointly inhibit the expression of fos gene. The reduction of cor2 leads to an increase in the expression level of fos, which enhances the fate of blood vessels and increases the inner diameter of arteries. Elevated fos will promote the expression of vegfd, activate the Notch signaling pathway and inhibit the production of hematopoietic stem cells.

　　The research results clarified the mechanism of Ncor2 in the development of zebrafish hematopoietic stem cells, deepened the understanding of the development process of hematopoietic stem cells, and provided a theoretical basis for the proliferation of hematopoietic stem cells in vitro.

　　PhD student Wei Yonglong in the research group is the main author of the paper, and researcher Liu Feng is the author of the correspondence. it is. This research work is supported by the National Major Basic Research and Development Program, the Natural Science Foundation of China, the 100 Talent Program of the Chinese Academy of Sciences, and the Strategic Leading Science and Technology Program of the Chinese Academy of Sciences.