动物实验,miR-100 z-bo 2020-09-26 466

　　The research team led by Professor Zhang Xiaobo of Zhejiang University found that miR-100 is a kind of anti-appropriate miRNA, which can achieve anti-sepsis through targeted regulation of trypsin gene. Using miR-100 antisense oligonucleotides to down-regulate the expression level of miR-100 may be a strategy to prevent viral infection.

　　Crayfish (Marsupenaeusjaponicus) is an important aquatic product species in Japan. Its reproduction process is often affected by virus infection, causing huge losses. As you know, micrornA (miRNA) is a 21-25t long non-coding RNA that can control the process of programmed cell death or apoptosis. However, the complete theory of miRNAs related to apoptosis is not fully understood.

　　A research team led by Professor Zhang Xiaobo of Zhejiang University conducted a detailed study of miRNAs related to shrimp cell apoptosis, and the results were published in the June issue of the journal "Fish and Shellfish Immunity".

　　Researchers analyzed the miRNA expression profile of shrimp infected with white spot syndrome virus (WSSV) using a custom-made shrimp miRNA chip (the custom miRNA chip project was completed by Lianchuan Biotechnology) and found that 199 miRNAs are involved in cell apoptosis, 8 of them miRNA is highly conserved in the evolution of animals. Later, the researchers conducted a loss of function experiment (LOF) on shrimp and found that miR-100 is an anti-appropriate miRNA, which can achieve anti-apoptosis through targeted regulation of trypsin genes. The results showed that, compared with the control group, silencing the expression of miR-100 can increase the apoptotic activity of shrimp blood cells, and further reduce the virus genome copy number and the mortality of virus infected shrimp. I'm. This study found that the miR-100-trypsin signaling pathway plays an important role in antiviral immunity by regulating cell apoptosis. Using miR-100 antisense oligonucleotides to down-regulate the expression level of miR-100 may be a strategy to prevent viral infection.