动物实验,血管增生 z-bo 2020-09-28 410

　　Retinal and choroidal angiogenesis is usually the loss of vision in severe eye diseases such as retinopathy of prematurity (ROP), diabetic retinopathy (DR), age-related macular degeneration (AMD) and retinal vein occlusion (AMD). Is the main reason. VO) and other ROPs are the main causes of blindness in Western children. Treatments include cryotherapy or laser ablation.

　　Angiogenesis-promoting factors play an important role in the pathogenesis of proliferative retinopathy, and vascular endothelial growth factor (VEGF) has been studied the most. Therefore, treatment of diseases with anti-VEGF active substances can be used as an alternative to traditional laser surgery. Although many VEGF blockers have been developed, including pegaptanib, ranibizumab, afribercept, and bebasizumab, the complexity of the VEGF pathway has many relative side effects. Although the study of retinal vascular proliferation has been extensively studied in various mouse models, especially pharmacological studies. However, due to the high cost of using mammalian models and the limitation of reproduction, it cannot be used as a tool for large-scale screening. Zebrafish is a typical tropical fish with good light conditions, daytime activities and visual functions. The eyes of zebrafish begin to develop 11 hours after fertilization (primitive optic nerve appears), and are almost fully developed 72 hours after fertilization (cardiogram can be recorded), and are related to visual function 6 days after fertilization. Some reactions are complete. Zebrafish has been proposed as an effective alternative animal model to study the mechanism of angiogenesis, including basic physiological and pathological studies. Through this principle, Ring Tokusei successfully found a model of zebrafish eye blood vessel growth, and successfully used Aescines and digitalis and bevacizumab positive drugs to validate the model. By observing the area of blood vessels in the eyes of the transgenic green fluorescent zebrafish strain of zebrafish, the pathology of the eye tissue and the expression of the hif gene, we can screen effective anti-vascular drugs.