动物实验,脊髓损伤修复 z-bo 2020-09-29 366

　　Objective: To use zebrafish spinal cord injury model to study the effects of water temperature changes on spinal cord injury repair, and to detect the changes in the number of cells in the damaged area and the expression of related genes in the brain during spinal cord repair.

　　Method: Use surgery to establish a zebrafish spinal cord injury model. Observe the changes in swimming ability after spinal cord injury by changing the water temperature of zebrafish life. Vibration section technology and immunohistochemistry were used to observe the number of cells at various points in spinal cord injury. Environmental changes; real-time fluorescence quantitative PCR method to analyze the differences in the expression of related genes gdnf and nos in the brain.

　　Results: In the non-operated group and sham-operated group, the water temperature of 28°C, 30°C and 32°C will not change the swimming ability of zebrafish (P\→0.05); the water temperature of 32°C can make zebrafish The fish became the spinal cord injury group. The ability of the 28 zebrafish to resume swimming under the water temperature of °C and 30 °C was significantly improved (P0.05), but at the same time it was significantly lower than the sham operation group on the 14th day (P\u003c0.05) ).

　　Conclusion: Properly raising the temperature of the aquatic environment can help zebrafish recover from spinal cord injury.