心肌梗塞,干细胞 z-bo 2020-09-30 351

　　As an emergency medical event caused by severe cardiovascular disease, myocardial infarction (MI) causes permanent life-threatening damage to the heart. In this new study, researchers from research institutions such as the City University of Hong Kong and the Catholic University of Korea have developed two methods that can use two kinds of stem cells to regenerate cardiomyocytes and the cardiovascular system simultaneously. This discovery brings hope to the development of a method for the treatment of myocardial infarction (MI heart) heart. Once successful, this treatment method will become an existing complicated and dangerous heart transplantation method for critically ill patients. Expected to be used. alternative plan. Myocardial infarction is a fatal disease caused by insufficient blood supply to the coronary arteries of the heart muscle. It causes the permanent loss of cardiomyocytes (CM) and the formation of scar tissue, which can cause irreversible damage to heart function and even lead to heart failure. There are limited treatment options for severe myocardial infarction and advanced heart failure, and heart transplantation is the last option. However, this is risky, expensive and limited by the right donor. Therefore, stem cell-based therapy has become a promising treatment option.

　　Dr. Pan Jiyuan, a stem cell biologist from the Department of Biomedicine, City University of Hong Kong, is studying a new stem cell-based heart regeneration therapy. "The heart is an organ composed of myocardium and blood vessels. It is necessary to provide oxygen and energy to the muscles of the heart. After a myocardial infarction occurs, the muscles and cardiovascular system of the heart will be severely damaged. Considering factors, therefore, treatment strategies should Focus on repairing two at the same time, and before that, these strategies only focused on repairing one. Based on this, Dr. Ban and his collaborators developed two methods: human bone marrow mesenchymal stem cells (hMSC) and human induced pluripotency Cardiomyocytes derived from stem cells (hipsC-CM). The purpose is to use two main types of stem cells to regenerate myocardium and cardiovascular system:

　　In this new study, hMSC has excellent paraclinical cell activity, angiogenesis and endothelial cells. The reason for using it is that it can secrete excellent proteins to promote survival; the reason for using HipsC-CM is its compatibility with humans: the primary myocardium is a heart-specific gene, structural protein, and ion channel. Expression, and more importantly, there are similarities in spontaneous contraction

　　We first studied the effects of two different stem cells on heart repair

　　Previous research has made hiPSC-CM or hMSC beneficial to myocardial infarction. This is the first study to simultaneously study the role of these two different stem cells in heart repair. The researchers used two methods: hMSC and hiPSC. -CM is delivered by two different routes: HipsC-CM is injected directly into the surrounding area of the rat heart, and a patch loaded with hMSC is implanted on top of the infarct area. It's like a bandage. These results indicate that this two-pronged approach can significantly improve cardiac function and enhance angiogenesis in MI hearts; the implanted hMSC load patch can lead to vascular remodeling. The microenvironment also improves the retention of hipsC-CM, and ultimately enhances heart function and restores damaged myocardium.

　　In addition, histological analysis revealed hMSC. The port of the loaded patch has been shown to facilitate the injection of hiPSC-. The function of CM is mature. It has a thinner rectangular shape and looks more organized when assembled. This is the source of mature adult cardiomyocytes. It is a type of morphological feature. The functional maturity of the hip C-CM injected into the myocardium is particularly important. .. This is because it can reduce the potential risk of arrhythmia. Arrhythmia is the irregular contraction of the heart, which is the main cause of sudden cardiac death.

　　Heart regeneration and other potential applications

　　Ban said: We believe that these two new methods may bring transformational and clinical benefits to the field of heart regeneration. Based on the same principle, this treatment may also be suitable for repairing other organs where multiple types of stem cells coexist, such as the brain, liver, and pancreas. "These researchers are currently tracking large animal models (such as pigs)