[Animal Modeling]-Behavioral Evaluation Method of Parkinson
动物造模,帕金森
z-bo
2022-02-02
744
1. Independent activity quantity reference
Kawai’s test method is to create a 30 cm x 30 cm x 15 cm plexiglass box with a 6 cm x 6 cm grid engraved on the bottom, and test in a quiet low-light environment. After allowing the animal to adapt to the environment for 10 minutes, calculate the number of grids moved in the trot and the number of standing time within 5 minutes, and take the average of 5 consecutive measurements.
2. Roller experiment
The roller experiment requires animals to maintain balance and move continuously on the roller. This is a widely used experiment to detect motor coordination. The diameter of the roller is 6cm, the speed is 20r/min, the detection interval after 5 applications is 1min, the continuous measurement is 5 times, and the average value is taken.
3. Climbing pole experiment
O According to Ogawa's test method, a straight wooden pole with a diameter of 18 cm and a height of 60 cm was made. At the top of the pole is a small wooden ball covered with gauze to prevent the mouse from slipping. Place the rat head up on the top of the rod and record twice. The time the animal starts to move and the head is completely lowered, and the time the animal lowers to the bottom of the rod. Each test interval is 1 minute, a total of 5 tests are performed, and the average value is taken.
Four suspension experiments
According to Kurihara's test method, a self-made plexiglass suspension test box with a diameter of 115mm and a height of 30cm from the ground is placed horizontally on a metal bar of 1cm, and then the animal is placed on the metal bar. I did not sit down. In the experiment, a wooden sh was suspended on a metal rod, and the landing time was recorded and scored according to the following criteria: 0-4 seconds, 1-5-9 seconds, 2-10-14, seconds, 3?15?19 second. 4 points for 20-24 years old, 5 points for 25-29 years old, 6 points for 30 years old and above. The interval between each test is 1 minute, a total of 5 tests, and the average value is taken.
go. Coefficient of variation
Some behavior detection methods use different units, so by calculating the coefficient of variation (coeffcient of variation, cv) of the five detection results of each tree diagram, we obtain 10 tree diagrams for each detection method. I will. Compare the coefficients of variation x and s between behavior detection methods, and compare the stability of the results measured by different methods. For each detection method, sequentially calculate the x and s of the first to fifth test results of 10 sh, and calculate the CV of each result to reflect the change trend of the first to fifth test results. I forget
6. Dry maze experiment
Rodents have not done any cognitive experiments before. Three days before the experiment, the wooden sh was able to explore the odor traces remaining on the board every day for a total of 60 minutes to adapt to the new "object" in the cage. A total of 10 experimental stages were tested. The experiment was conducted once a day for 5 consecutive days at a central 2d interval, and then another 5 consecutive experimental periods. The experiment in each stage includes 4 visual spaces and 1 spatial experiment, with an interval of 10 minutes after each experiment stage. During the experiment, the animals were observed through a monitor and their behavior was recorded. Test animals for a maximum of 90 minutes from 13:00 to 18:00. During the visual space experiment, 5 new random sequencing holes were pasted from 23 holes every day and put on a small slice of almond as bait. Place the well plate in the cage until the animal has walked through all the holes containing food. Afterwards, remove the orifice plate from the cage, attach the bait to it, and return the orifice plate to the cage. In the space experiment, the hole cover logo was deleted. In these four visual space experiments and one space experiment, the marked holes used are the same. The order of the holes will be changed in the next day's experiment. Count the number of holes and record the order in which the animals visit them until all five holes with food are found. If the wood sh crawls to the animal cave and uses its forehead or nose to actively move the cover, it is counted as a hole. Entering an unequipped bait hole is the wrong choice. Revisiting the hole with bait is defined as repeated selection. In addition, measure the time it takes for each animal to explore the cave with five baits. After preparing three PD models, the experiment was used to determine the changes in the spatial cognitive ability of rodents. The above-mentioned quantitative behavioral observation methods provide some more accurate and objective methods for behavioral research in rodent models of Parkinson's disease, and also improve the overall objectivity of the experiment.
