干细胞移植 z-bo 2020-10-07 281

　　Human clinical trials have begun to show that stem cell-based therapies are expected to enhance myocardial function and treat other diseases. However, in addition to observing clinical results, there is currently a lack of reproducible methods for evaluating the efficacy of cells transplanted into target organs (to produce stem cells). Sensitive, non-invasive tool.

　　Researchers at the University of Maryland School of Medicine (UMSOM), the University of Pennsylvania (University of Pennsylvania) and Emory University believe that blood tests can be used to track the effectiveness of transplanted stem cells. Their goal is to monitor exosomes by analyzing the small cell components secreted by transplanted stem cells into the recipient’s blood. After transplanting two types of human cardiac stem cells and monitoring the circulating exosomes, the theory was tested in rodent models of heart attacks or myocardial infarction. Researchers have discovered that circulating exosomes deliver cellular components to target cardiomyocytes, leading to heart repair. The research results were published in the journal Science Translational Medicine. "Exosomes contain signals from the cells from which they are derived, such as proteins, nucleic acids, and microribonucleic acid (microRNA). These signals affect the recipient cells and provide necessary target organs. It may change or regenerate," Children's Hospital of the University of Maryland Said Dr. Sunjay Kaushal, Director of Cardiac Surgery and Professor of Surgery at UMSOM. "Currently, there are tools to determine whether stem cell therapy is effective for a patient. It can be used for any stem cell therapy, not just heart therapy."

　　Through blood tests, researchers can determine the condition after myocardial infarction. I monitored the transplant. Seven days after transplantation, human cardiomyocyte-derived cells (CDC) and cardiomyocyte precursor cells (CPC) from the mouse heart were compared with plasma exosomal concentrations.

　　After purifying the exosomes of CDC/CPC, the researchers found that the exosomes contained miRNAs related to myocardial recovery. In addition, they found that the content of CPC and CDC produced by culture is different from the content of exosomes produced by live transplanted cells.

　　"Our research should be seen as the first step in understanding the function of stem cells, but what is important is that cells that are identified as responsive can alter gene expression, behavior, and secretion. This is what I did," Assistant Professor of Surgery at UMSOM Dr. Sudhish Sharma, the co-author of said. "By using these biomarkers, we can understand the mechanism and extent of recovery."