Hyperlipidemia animal model
动物模型,高脂血症
z-bo
2020-07-25
712
[Program] The most commonly used experimental animals are rabbits and rats.
Rabbit hyperlipidemia feed:
Formula 1: 15% egg yolk powder, 5% lard, 80% alkaline feed, 0.5% cholesterol. After 3 weeks of feeding, after a certain amount of serum cholesterol rises, a certain amount of serum cholesterol cannot be added, and the feed containing egg yolk powder and lard can be fed for 3 weeks for a total of 6 weeks. The incidence of aortic plaque is 100%, and serum cholesterol can increase to about 2000 mg/dl. Formula 2: once a day, 6 times a week, once a day with basic feed and 0.5 grams of cholesterol (capsules directly filled and fed).
Wistar rat hyperlipidemia diet:
Formula 1: Cholesterol 2%, propylthiouracil 0.2%, sodium cholate 0.3%, lard 7.5%, basic diet 90%, high fat can be taken for 42 days to establish a disease model. Formula 2: 3% cholesterol, 10% lard, 0.2% methionine, 86.8% basic diet, 20 days of high-fat diet can establish a hyperlipidemia model.
SD rats high-fat diet:
Cholesterol 1%, cholic acid 0.5%, methylthiouracil 0.2%, lard 2%, soybean 5%, egg and fish meal 1%, egg yolk powder 2%, a hyperlipidemia model was established after 4 weeks of feeding, accounting for 88.3 %.
The above modeling methods are free for animals to use. Although it is simple, the intake of each animal cannot be guaranteed. Therefore, fat milk tube feeding can be used. Cholesterol 10%, lard 20%, sodium cholate 2%, methionine 1%, Tween 8020%, propylene glycol 30%, water 100% to make a fat emulsion, gavage 10ml/(kg·d) take medicine 10 Days can cause hyperlipidemia models.
[Matters needing attention] The type of animal directly affects the replication model. The experimental hyperlipidemia study is a long-term experiment. The feeding and management of animals is very important. If rabbits are used as experiments, the feed can be used once a day. The quantity is based on much more food. A small amount of green vegetables should be added every day. .. [Result analysis] Animals have high absorption of exogenous cholesterol (up to 75%-90% for rabbits, 80% for dogs, and up to 40% for rats), while the ability to remove hyperlipidemia is very low (rabbit). Hyperlipidemia (after intravenous injection of cholesterol) lasts for 3-4 days, in rats for 12 hours, and in dogs in between. Feeding a high-fat diet will form an animal model of hyperlipidemia. Generally, the formula of a high-fat diet is similar, but the higher the fat content, the shorter the time required for modeling and the higher the proportion of lard. This is important; the antithyroid metabolites methionine or propylthiouracil can make the model successful and the effect remains the same; if the cholesterol content in the formula is high, cholate may be used to increase absorption. Best; while the formula is complete, the model time of SD rats is shorter than that of Wistar rats.
