【Animal model】-Rotenone-induced Parkinson
动物模型,帕金森病
z-bo
2022-01-13
494
1. Modeling material animal: Lewis rat, male, weighing 260-280g; drug: rotenone. 2. Modeling method Rotenone (1.0 g/L) was dissolved in a mixed solvent of dimethyl sulfoxide: normal saline (2:1), the normal control group was injected subcutaneously with a mixed solvent without rotenone, and the model group was injected subcutaneously with rotenone [ 1.0 mg/(kg?d)], divided into two injections, once in a row, every morning and evening at 8:00, for 30 consecutive days, once a week.
3. Modeling principle Rotenone (rotenone) selectively damages rat SN dopamine neurons and produces unique Lewy bodies, but does not affect cholinergic neurons.
4. After modeling, the rats in the control group gained weight, and the rats in the model group initially lost weight, and the weight gradually returned to a constant level, and it did not change over time. It was always lower than the control group.
5. After modeling, count 10 pathological and biochemical changes under an immunofluorescence microscope. Compared with the control group, the substantia nigra TH-positive cells in the model group were significantly reduced, and the remaining neurons and nuclear membrane were covered. Under the microscope, issl staining can reduce the number of substantia nigra neurons in the model group, enrich the surviving neurons and accompany the proliferation of glial cells.
