帕金森病,移植人干细胞 z-bo 2020-10-09 266

　　After being injured by trauma, stroke or degenerative disease (such as Parkinson's disease), the mature brain is very poor in self-repair. Stem cells with unlimited adaptability provide hope for better nerve repair. However, the complexity of precise brain coordination hinders the development of clinical treatments.

　　New research aimed at overcoming these obstacles includes the University of Wisconsin-Madison, the Chinese Academy of Sciences Center for Excellence in Brain Science and Intelligent Technology, the Chinese Academy of Sciences, the Shanghai Center for Brain Science and Brain-like Research, and Duke University researchers at the National University of Singapore School of Medicine confirmed that, Stem cell therapy can prove this concept in a mouse model of Parkinson's disease. They found that the neurons produced by human embryonic stem cells (ESC) have successfully integrated into the correct areas of the brain and established connections with the endogenous neurons of these mice and restored motor function.

　　The key is identity. By carefully tracking the fate of transplanted stem cells, these authors discovered that the identity of these cells in Parkinson's disease refers to dopamine-producing neurons (that is, dopamine-producing neurons, also called dopaminergic neurons). The connections and functions they create. With the increasing use of stem cells to generate dozens of unique neurons, this study shows that neural stem cell therapy is a viable goal. However, further research is needed to convey the results of the mouse study to humans. Zhang Suchun, a neuroscientist at the University of Wisconsin-Madison, said: “Our brains are precisely connected by very specific nerve cells at specific locations to perform all complex movements. It can be done, everything depends on it, through connection Neural circuit damage caused by specific cell types usually affects specific brain areas or specific cell types, causing neural circuits. In order to treat these diseases, these neural circuits need to be repaired.

　　"To repair, in a mouse model of Parkinson's disease, dopamine-producing neurons will die of Parkinson's disease. Therefore, these authors first induced the differentiation of human ESCs into dopamine-producing neurons. They transplanted these newly generated neurons into the midbrain of these mice, which is the brain area most affected by Parkinson's degeneration. A few months later, after having time for these newly generated neurons to integrate into the brain, the exercise capacity of these mice improved. Through careful observation, Zhang and his team were able to observe that these transplanted neurons extend a long distance and are connected to the motor control areas of the brain. They also establish contact with the regulatory regions of the brain that enter new neurons and prevent them from being overstimulated. The connection between the input and output of these two groups of transplanted neurons is similar to the neural circuit established by endogenous neurons. This only applies to neurons that produce dopamine. Similar experiments were performed using neurons that produce the neurotransmitter glutamate, which is not involved in the development of Parkinson's disease. The results showed that the transplantation of these glutamate-producing neurons could not repair the motor circuits of these mice, and the importance of neuron identity repairing damage. In order to finally prove that these transplanted dopamine-producing neurons repaired neural circuits damaged in Parkinson's disease, these researchers inserted a switch gene into human embryonic stem cells. It is achieved that when these cells are exposed to specially designed drugs in the diet or through injection of drugs, these on/off switches will increase or decrease the activity of these cells. When these stem cells were turned off, the improvement in the exercise capacity of these mice disappeared, indicating that these stem cells are essential for restoring the brain caused by Parkinson's disease. New research also shows that this gene conversion technology can be used to fine-tune the activity of transplanted cells to optimize treatment. Over the years,

　　Zhang's research team and other researchers have developed methods to transform stem cells into different types of neurons in the brain. Each neurological disease or injury requires its own specialized nerve cells for treatment, but the treatment plan can be similar. Zhang said: "We use Parkinson's disease as a model, but the principles are the same for many different neurological diseases."

　　This research has personal significance to Zhang. As a doctor and scientist, he often receives letters from families eager to treat neurological diseases or brain injuries. This is also an experience he can sympathize with. Six years ago, Zhang broke his neck in a bicycle accident. He was half paralyzed when he woke up in the hospital. The first thing he thought of was stem cells-he has been studying for many years-how it can help his recovery.

　　After years of physical therapy, Zhang still believes that the right stem cell therapy may help people like him and the family he will hear in the future.

　　For this reason, Zhang's team is currently testing similar treatments for primates. This is the first step towards human clinical trials. He said: "There is hope, but we need to take steps gradually."