动物实验,肠道菌群 z-bo 2020-10-12 564

　　Objective: To investigate the effect of plant sensitive signal molecule N-acyl homoserine lactone (AHL) on the composition of the intestinal flora of mice, and to provide a theoretical basis for disturbing the intestinal flora under infection.

　　Method: 3-oxo-C10-HSL molecules were injected intraperitoneally into mice, and samples were taken from the duodenum and air intestines of mice by cotton swab smears 24 hours later and used on the Illumina Hiseq2500 high-throughput sequencing platform sampling. 16SrDNA sequence, and analyzed the classification and diversity of the flora.

　　Results: A total of 22 flora and 109 bacterial genera were detected in the intestines of experimental mice; the fimbriae (→50%) of the control group and the 3-oxo-C10-HSL treatment group and although it is a Proteus (20%), but there are significant differences in the composition of the two groups at the genus level. The main bacteria in the control group were Staphylococcus, Escherichia coli-Shigella, Lactococcus, Pseudomonas 3-oxo-C10-HSL. The main bacteria in the treatment group were Enterococcus, Escherichia coli-Shigella and Staphylococcus. Analysis of diversity and abundance indicated that 3-oxo-C10-HSL treatment was used. The diversity of intestinal flora in this group has increased, the abundance of main dominant bacteria (such as E. coli) has increased significantly, and the abundance of airborne colonies is the highest.

　　Conclusion: AHL has little effect on the composition of mouse intestinal flora, but it has a great impact on the structural changes of certain groups in plants.