动物实验,环磷酰胺 z-bo 2020-10-12 381

　　Objective: Inject cyclophosphamide intravenously into pregnant rabbits, observe its toxic effects and performance on pregnant rabbit embryos and fetuses, and provide reference for selecting cyclophosphamide as a positive control.

　　Methods: The pregnant rabbits were randomly divided into a control group [0.9% sodium chloride injection, GD11 (GD, pregnancy day), intravenous injection] and a cyclophosphamide group (cyclophosphamide 30 mg/kg body weight, GD11, intravenous injection) ). Divided. GD28 euthanized a pregnant rabbit and collected the fetus through a royal incision to examine the appearance, internal organs and bones of the fetus.

　　Result: The cyclophosphamide group did not significantly affect the rabbit's reproductive function and embryogenesis, and may cause the appearance, internal organs and bone deformation. The rate of fetus with cleft malformation is 32.95%; the visceral variability is 10.23%; the bone malformation is 23.37%.

　　Conclusion: In New Zealand, 30 mg intravenously? kg-1 cyclophosphamide without using GD11 can cause fetal malformations. The type and incidence of deformity are appropriate and can be used as a positive control for reproductive testing.