动物实验,蛋白E敲除小鼠 z-bo 2020-10-12 347

　　Objective: To study the influence of western diet and standard diet on hematology and pathology of ApoE protein E knockout mice (ApoE-/-).

　　Method: ApoE-/- was fed a Western diet and a standard diet to 3-month-old mice, and the peripheral blood and aortic arch of the mice were collected at 3, 4, and 5 months. An automatic blood analyzer was used to detect the blood lipid level, and the formation of atherosclerotic plaque was observed by sectioning and staining.

　　Regarding blood lipids, total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) in the serum of mice fed on a Western diet and a standard diet, C57 is lower than that in the control group. mouse. Except for HDL-C in mice raised for 4 months, the blood lipid index of the western diet group was significantly higher than that of the standard diet group. At the same age, in terms of pathological sections and staining of aortic arches, the aortic plaques of mice in the Western diet group were obvious, and the incidence of AS was significantly increased.

　　Conclusion: Western and standard diet can induce ApoE-/- mice to show AS symptoms, and Western diet has a more obvious inducing effect.