动物实验,miRNA-144 z-bo 2020-10-12 373

　　Objective: To analyze the anti-tumor effect of microRNA-144 (miRNA-144) gene overexpression on liver cancer mice and its influence on the intestinal flora. How to establish ascites liver cancer transplanted into mice.

　　Method: Observe the effect of overexpression of miRNA-144 gene on the general condition of liver cancer mice, the histopathological morphology of liver and intestine, the richness and diversity of intestinal flora and the intestinal barrier function. Real-time fluorescent quantitative polymerase chain reaction and western blotting are used to determine the status of occludin, ZO-1, miRNA-144 mRNA and occludin, ZO-1 protein.

　　Results: The overexpression of miRNA-144 improved the general condition of liver cancer mice, inhibited the development of liver cancer, improved the intestinal microeconomic environment and intestinal mucosal barrier function, blocked the colon tissue, and up-regulated ZO-1 and miRNA- 144 mRNA and occludin. , ZO-1 protein expression.

　　Conclusion: The overexpression of miRNA-144 may inhibit the development of primary liver cancer and improve the microeconomic environment and intestinal mucosal function of the mouse intestine. This mechanism may be related to the up-regulation of occludin, ZO-1 mRNA and protein expression.