神经认知障碍,动物实验,肠道菌群紊乱 z-bo 2020-10-12 379

　　Perioperative neurocognitive disorder (PND) refers to the common anesthesia in elderly patients and the changes in mental activity, personality, social skills and cognitive abilities of patients after surgery. Clarifying its pathophysiological mechanism helps to formulate effective preventive measures. The diversity and stability of the intestinal flora of elderly patients are significantly reduced. And surgery, especially gastrointestinal surgery, exacerbated the imbalance of intestinal flora. In a recent study, "gut-brain dialogue" mediated by "gut microbiota-gut-brain axis" is related to immune-mediated central nervous system diseases (Gilan-Bar syndrome, Giran-Bar syndrome) . It has shown a two-way communication function between the internal microbiota and the brain. Multiple sclerosis and neuromyelitis optica) and non-immune-mediated central nervous system diseases (depression, anxiety, autism, etc.) play an important role.

　　60SPF-class healthy male C57BL/6J mice, such as Wang Guangshi, Wu Xiaoxiu, and Han Xinjing, People’s Hospital of Zhongzhou University, to evaluate the role of intestinal microflora in perioperative neurocognitive impairment in elderly mice, age 18 months , Using random number table method to divide into 4 groups (n = 15): control group (C group), surgery group (O group), surgery + Lactobacillus rhamnosus group (OL group) and surgery + fecal bacterial transplantation group ( OF group). O, OL and OF groups underwent exploratory peritonectomy. In the PL group, 200 μl of Lactobacillus rhamnosus was administered intragastrically immediately after surgery, once a day for 10 consecutive days. Mice can get about 0.2×109 CFU of probiotics every day. In the first 7 weeks of the OF group, a broad-spectrum antibiotic mixture (ampicillin and sulfa 1.5 g/L, vancomycin 500 mg/L, ciprofloxacin 200 mg/L, imipenem and cilastatin 250 mg /L) and metronidazole 1 g/L) Except for the drinking water of mice, use sterile tap water 72 hours before surgery. After the operation, 200μl of fecal bacterial filtrate was administered into the stomach once a day for 10 days. Ten days after the operation, 5 mice in each group were sacrificed, and the air and ileum vascular permeability were tested by the Evans blue leak test. On the 10th day after the operation, 5 mice in each group were slaughtered. Small intestine, hippocampal tissue and orbital venous blood samples were collected, and IL-6, IL-17, TNF-α, IFN-γ, IL-4, and IL-4 were collected by ELISA. , IL- is detected. 10 levels. Ten days after the operation, five mice were collected in each group, and the cognitive function of the mice was measured using the Morris water maze test. The results showed that compared with group C, air and ileum permeability in group O increased, and the levels of IL-6, IL-17, TNF-α and IFN-γ in the small intestine, peripheral blood and hippocampus increased. Compared with the IL-O group, the air and ileal vascular permeability of the OL and OF groups increased and decreased, the small intestine and peripheral blood decreased, the IL-10 level decreased, the swimming distance and escape lengthened the waiting time and shortened the target quadrant The time spent in (P0.05). In addition, Kaiba's IL-6, IL-17, TNF-α and IFN-γ levels decreased, IL-4 and IL-10 levels increased, swimming distance and escape latency were shortened, and the target quadrant. The stay time has been extended (P\u003c0.05).