动物实验,牛磺酸 z-bo 2020-10-12 341

　　Objective: To observe the effect of taurine on the expression of blood hormones and IGF genes in pregnant rats with intrauterine growth restriction.

　　Method: Take 2 healthy SD rats, 2 male and female, randomly select pregnant rats according to the order of pregnancy, normal diet group (C), low protein diet group (M), low protein diet + taurine group (MT), Ordinary feed + taurine group (T). There are 10 pregnant rats in each group. To establish the model, the M and MT groups received a low-protein diet during pregnancy. On the 12th day of pregnancy, pregnant rats in the C and M groups were forcibly given water, and the MT and T groups were given taurine 300 mg/(kg·D). The weight gain of pregnant rats was detected weekly, and samples were taken on the 21st day of pregnancy, and the weight, number and number of fetal rats were recorded. The Elisa method detects serum growth hormone (GH), insulin growth factor 1 (IGF-1) and insulin (INS) in pregnant rats. Detection of tyrosine (T3 and T4) levels, pregnant mouse liver growth hormone receptor (GHR), insulin growth factor 1 (IGF-1), insulin growth factor binding protein 1 (IGFBP-1) and insulin growth fluorescence quantitative PCR Methods To detect the expression level of factor binding protein 3 (IGFBP-3) mRNA.

　　Result: The weight, number and deformity of the fetal mice in the taurine group were significantly improved, the weight of pregnant mice was significantly increased (P \u003cu003c0.05), and the blood INS and T4 were significantly increased. (P\u003c0.05), the expression of IGF-1 and IGFBP-3 mRNA in the liver was significantly increased (P\u003c0.05), while IGFBP-1 mRNA was significantly decreased (P\u003c0.01).

　　Conclusion: The addition of taurine can improve thyroid function and GH/IGF-1 axis defects in low-protein pregnant mice, and may play an important role in reducing fetal uterine growth restriction.