动物实验,Ⅰ型干扰素受体缺失 z-bo 2020-10-12 344

　　Purpose: To study the effects of two type I interferon receptor deletions on in vitro fertilization in mice and optimize the conditions of in vitro fertilization.

　　How to perform in vitro fertilization and embryo transfer in two type I interferon receptor-deficient mice (IFN-αR-/-, IFN-α/βR-/-) and background wild-type mice (C57BL/6), Record five mice per group of superovulation, three replicates and related data to analyze whether the lack of interferon receptors affects the in vitro fertilization of mice. In vitro fertilization hybridization experiments were performed on two type I interferon receptor-deficient mice and C57BL/6 mouse partners, 5 mice per group, 3 times per group, male and female type I interferon receptor deficient mice . The effect of fertilization on the spouse. At the same time, we will explore technical methods to optimize IVF conditions and increase fertilization rate. 5 mice per group, repeat 3 times. Results The average in vitro fertilization rate of two type I interferon receptor-deficient mice was lower than that of the background strain C57BL/6 mice, and the difference between the two groups was significant (P\u003c0.05). The in vitro fertilization rate of interferon α receptor-deficient mice was higher than that of interferon α and β receptor double-deficient mice, and the difference between the two groups was statistically significant (P\u003c0.05). The in vitro fertilization rate of sperm in two type I interferon receptor-deficient mice and C57BL/6 mouse egg cells was higher than that of egg cells and C57BL/6 mouse sperm. The difference between the two groups was significant (P\ u003c0. 05). ). Extending the fertilization time of sperm to 1 hour or adding 1 mmol/L reduced glutathione (GSH) to the fertilization and fertilizer solution can increase the in vitro fertilization rate and the difference between the corresponding groups is significant (P\u003c0.05 ).

　　Conclusion: The lack of type I interferon receptors will lead to a decrease in the in vitro fertilization ability of corresponding mouse strains, and the egg cell effect is more important than the sperm effect. The in vitro fertilization rate can be improved by appropriately extending the fertilization time of sperm or changing the fertilization amount and fertilizer composition.