动物实验,脑缺血模型 z-bo 2020-10-13 364

　　Objective: To observe the feasibility of electrocoagulation on the middle cerebral artery in mice, cause cerebral artery occlusion, and establish a model of cerebral ischemia in mice.

　　Method: Use electrocoagulation to directly occlude the middle cerebral artery (MCA), and create an adult male Balb/c mouse cerebral ischemia model (model group, n = 20). At the same time, create the same batch of Balb/c mice. Sham operation group (sham operation group, n = 20) mice with the same skull incision but no electrocoagulation and middle cerebral artery occlusion. The neurological function score (mNSS) was used to evaluate the nerve damage in the model and sham operation groups at 24 and 72 hours after surgery.

　　Result: Twenty-four hours after the operation, a slice of the brain tissue of the injured part of Balb/c mice in the model group was taken, and hematoxylin-eosin was stained. Microscopic examination revealed ischemic brain tissue interstitial edema with cavity formation and local brain tissue structure. It becomes a loose pale color, the number of nerve cells is greatly reduced, the cell body of nerve cells shrinks, and the outline of some cells becomes unclear. Different degrees of cell degeneration and necrosis, nuclear abscesses and nuclear loss. After 24 hours, the neurological deficit score of the model group was significantly lower than that of the sham operation group (P\u003c0.05), and this difference can be maintained until 72 hours after surgery.

　　Conclusion: Electrocoagulation can successfully create a mouse model of local cerebral ischemia in Balb/c mice.