复发性口腔溃疡动物模型 z-bo 2020-10-13 404

　　[Modeling mechanism]: Recurrent oral ulcers are superficial ulcers that develop in the oral mucosa. The lesion begins with local mucosal congestion and edema, accompanied by military red spots and obvious burning pain, and then takes a round or oval shape with a diameter of 5 mm. Based on the above characteristics, this model will reproduce similar pathological damage caused by chemical combustion.

　　[Modeling method]: When establishing a recurrent oral ulcer model due to chemical burns, animals such as New Zealand rabbits, rats, guinea pigs and mice can be selected. At the same time, you can choose different parts of the lips, cheeks and tongue mucosa according to your needs. The specific method is as follows. According to the selected animal, the animal is anesthetized using an appropriate method of anesthesia to expose the mucosa and cause ulcers. The prefabricated filter paper with a diameter of 5 mm and a thickness of about 1 mm is immersed in 50% glacial acetic acid and placed on the mucous membrane for 60 seconds. After removal, a white lesion with a diameter of 5 mm appeared on the mucosa in this area. After 24-48 hours, enter the area. An ulcer with a diameter of 5 mm may appear on the mucosa.

　　[Model Features]: The model operation method is simple, easy to implement, highly reliable and easy to repeat. Suitable for large animal experiments. Although the etiology and recurrent oral ulcers are different, the pathological changes are similar to humans. Certain drugs that promote oral mucosal healing and anti-inflammatory drug screening models by observing the healing process provide a scientific basis for clinical local administration.

　　[Model Evaluation and Application]: Commonly used chemical ablation chemicals are phenolic acid 90%, glacial acetic acid 50%, hydrochloric acid 1% and phenol 900g/L. Among them, 90% of phenolic acid is chemically unstable and easy to crystallize and precipitate. The induced ulcer is not constant, the chemical properties of glacial acetic acid are stable, and the induced ulcer is reproducible. The size, shape and depth of the ulcers are relatively consistent, which helps to compare research drugs. The model is simple and easy to use, suitable for large-scale animal experiments, can be used as a screening model for specific drugs and anti-inflammatory drugs that promote oral mucosal healing, and is simple and easy to perform for pharmacological research. Establish a feasible practical method. This model method is not suitable for animal experiments with recurrent oral ulcers.