实验动物 z-bo 2020-10-13 329

　　The purpose of grasping and fixing the animal correctly is to make the experiment go smoothly by damaging the health of the animal, affecting the observation index and preventing being bitten by the animal. The method of catching a fixed animal depends on the nature of the experiment and the type of animal. Before catching a fixed animal, you need to understand the general habits of different animals. In order to achieve the purpose of holding and fixing the animal correctly, you must be careful, bold and agile when holding and fixing the animal.

　　(1) How to hold and fix the mouse

　　The mouse is obedient and doesn't usually chew. To grab it, first grab the tail of the mouse with your right hand, lift it up, place it on the squirrel cage or laboratory table, and then pull it back and forth. When crawling, please use your left thumb and index finger to grasp the mouse’s ear and neck skin, place the mouse’s body in the center of your left hand, straighten the hind limbs, and then press the mouse’s tail with your ring finger. , Push the little finger back. Only legs. An experienced person can directly hook the rat’s tail with the little finger of the left hand, and then quickly pinch the skin behind the neck with the thumb, index finger and middle finger. This manual fixation method can be used for tube feeding of laboratory animals, subcutaneous, intramuscular, intraperitoneal and other experimental operations. The mouse needs to be fixed into a specific shape, such as anatomy, surgery, heart blood sampling, tail vein injection, etc. Through dissection and heart blood sampling, the animal is placed in the dorsal position (anesthetized if necessary), and then the forelimbs and hind limbs of the mouse are fixed on the wax plate with pins. For tail vein injection, the mouse tail vein injection holder can be used to fix it. First, choose a suitable bracket for the size of the animal, open the mouse tube cover, hold the mouse tail, and then place the animal's head in the mouth of the mouse tube. After entering the barrel, properly adjust the length of the mouse barrel to expose the tail, fix the barrel cover and perform tail vein injection or tail vein blood sampling.

　　(2) How to fix and fix the mouse

　　The method of fixing the mouse is basically the same as that of the mouse, except that it is sharper than the mouse. Not suitable for attack. Otherwise, you will bite your fingers. You can wear canvas gloves to avoid being bitten. You can also use the left hand fixation method for intraperitoneal, intramuscular, subcutaneous injection and gastroscopy. Pinch the mouse’s ear with your thumb and index finger, and place the remaining three fingers tightly behind the mouse and in the center of the left palm. Perform various experimental operations. You can also stretch your left hand and quickly grab it from behind. During the operation or dissection, it must be anesthetized or put to death in advance. Then tie the leg with a thin cotton thread and tie it to the rat fixed plate on the back. Fixed tail vein injection is the same as fixed mouse (just change the fixed frame). Just fix the mouse's box).

　　(3) How to hold and fix a guinea pig

　　guinea pigs are relatively timid and easily surprised, so they should not be strongly stimulated or scared, so they need to be held firmly, accurately and quickly. The common grasping method is to quickly tighten the back of the rat with the palm of the hand, hold the upper part of the shoulder blade, hold the neck with the thumb and indicator ring, and support the waist with the other hand.

　　(4) How to fix rabbit catch

　　1. Most experimental rabbits are locked in cages, so they are easy to grasp, but usually, you use your right hand to grasp the fur of the rabbit’s neck and lift it up, and then use your left hand to support the waist and abdomen, and most The weight is placed on the left hand. Concentrate to avoid harm to the animal during the grasping process. Do not grab your ears or belly.

　　2. Rabbit fixation is generally divided into three types: box shape, table shape and horseshoe shape. Fixing the box (suitable for blood sampling in rabbit ears and blood vessel injection in the ear. For blood pressure measurement, breathing, and other experiments and operations, the rabbit limbs should be fixed on the rabbit table. Knotted with thick cotton rope, straightened For limbs, tie the rope to the fixed wooden block around the rabbit shelf, fix the head with a fixing clip, or use a thick cotton rope on the rabbit to pick up the cut and tie it to the iron pillar of the rabbit shelf; horseshoe-shaped fixing pieces are mainly used The lower back, especially the skull experiment. When fixing, cut off the hair at the bottom of the eye hole to expose the protruding part of the joint, adjust the two ends of the fixing device, and nail it

　　type metal rod. It fits tightly in the groove under the protrusion and fixes the metal rod to an appropriate height. The horseshoe-shaped fixation device allows the rabbit to be placed on the back and abdomen, so it is a commonly used fixation method in research.