采集实验动物脑脊液 z-bo 2020-10-13 403

　　The cerebrospinal fluid can be collected by puncturing the spinal cord cavity, and small animals can also be collected directly from the large eye.

　　Cerebrospinal fluid is usually collected in rabbits by spinal puncture. The puncture site is located below the midpoint of the two vertebral lines in the seventh lumbar intervertebral space. After lightly anesthetizing the animal, fix its lateral position, bend the head and tail as far as possible to the waist, and cut the coat around the seventh lumbar vertebra. After disinfection, the operator uses the thumb and index finger of the left hand to fix the animal on the puncture site on the animal's back, and punctures the lumbar puncture needle with the right hand. When the animal's hind limbs beat, the needle reaches the spinal canal (svaraknoid space). Remove the core of the needle and check whether the cerebrospinal fluid is flowing out. If there is no cerebrospinal fluid outflow, the arachnoid membrane may not have penetrated yet. Gently adjust the direction and angle of needle insertion. If the cerebrospinal fluid flows too fast, insert a small needle core to prevent a sharp drop in intracranial pressure and herniation.

　　The cerebrospinal fluid of rats can be collected by directly piercing the blood in a large bottle. After the rat is anesthetized, the head is fixed to the alignment device. The head and neck were cut and sterilized, a vertical incision (approximately 2 cm) was made along the vertical axis of the skull, and the muscles behind the neck were separated straightly with scissors. To avoid bleeding, scratch the deepest muscles attached to the bones behind the skull to expose the large bottle in the hole. The cerebrospinal fluid is drawn directly from the large eye. After taking it out, sew the external muscles and skin. Sprinkle some sulfa powder on the incision to prevent infection. After the cerebrospinal fluid is collected, an equal amount of sterile normal saline should be infused to maintain the original pressure in the cerebrospinal cavity.