实验动物病理取材 z-bo 2020-10-13 441

　　1. Pathological examination should be carried out in different levels, with general appearance observation first, followed by necropsy observation, and then detailed light microscope inspection.

　　2. Usually select the tissue at the junction of normal and lesion, which includes the lesion itself and surrounding tissues.

　　3. When collecting materials from the same organs of animals in the control group, the selection of materials should be as consistent as possible.

　　4. When no obvious lesions are visible to the naked eye, the positions of specimens selected in each experimental group should be consistent.

　　5. The selected tissue should include all the hierarchical structures or important structures of the organs, such as the kidney should include cortex, medulla and renal pelvis.

　　6. For large and lobed organs, multiple parts should be selected according to different tissues. Small organs can be taken and fixed as a whole, such as lymph nodes, tonsils, and thyroid.

　　7. The contents of gastrointestinal specimens should be washed away to prevent the contents from affecting tissue fixation and causing autolysis.

　　8. The material taken should try to maintain the integrity of the naked eye specimen, not too thick or too thin, generally 3-5mm thick and 1.5-20m2 in size.

　　9. Do not squeeze when cutting tissue, use sharp knives, use less scissors, and do not use parts that have been pressed by the forceps.

　　10. It is necessary to be familiar with the collection of specimens and complete the entire process as quickly as possible, especially for tissues that are easily autolyzed, such as the intestine, brain, and glands.

　　11. The autopsy record should be objective and detailed, with a vivid description instead of the name of the diagnosed disease.

　　12. Animals in the control group and experimental group in the same experiment should be cross-examined, and various conditions and operations should be strictly unified, and various possible interference factors should be avoided as much as possible.