表皮细胞提取,新生鼠处死 z-bo 2020-10-14 341

　　method one

　　Generally, after wiping the whole body with alcohol, use scissors to beheaded and put to death, drip off the blood in the body, wipe off the blood on the neck with a cotton ball, and then fix it with a pin. It takes less than a minute and it will not affect the vitality of the cells. There is no stress response.

　　Method Two

　　Since it is necessary to separate cells, pay attention to aseptic operation

　　1. Disinfect the suckling mouse: soak the suckling mouse in 75% alcohol for a while, or wipe the whole body of the suckling mouse with alcohol.

　　2. Execution and bleeding: Use sterile scissors to cut the left armpit (corresponding to the position of the heart) of the suckling mouse and completely bleeding.

　　3. Material: The epidermal tissue taken is washed in sterile saline or PBS solution, and then processed for cell division.

　　Method Three

　　1. Soak the suckling rat in 75% alcohol for 5 minutes. (Basically, I feel dizzy after drinking, but also disinfected by the way)

　　2. Soak the new gelatin for 5 minutes (the suckling rat has been hung up and disinfect it again).

　　3. Take it out and make an incision from the neck, the tail and limbs, and the abdomen (be careful not to cut the peritoneum), and then the skin will come off. This is how the epidermal stem cells are taken, for reference only!

　　Method Four

　　1). Digest the epidermis again with 0.25% pancreatin at 37°C for about 8 minutes, add the same amount of medium containing 10% FBS as the pancreatin to terminate the digestion, and then fully pipette.

　　2) Centrifuge at 1000 rpm/min for 10 minutes, discard the supernatant and digested epidermis.

　　3) Add ESCs culture medium and pipet thoroughly to make a single cell suspension.