血脑屏障,动物实验 z-bo 2020-10-14 733

　　Introduction: The blood-brain barrier is composed of peers, the capillary wall of the choroid plexus, and the glial membrane formed by the glial cells located outside the wall. It acts as a natural barrier, allowing pathogenic microorganisms, toxic products and dye particles to enter the brain tissue and cerebrospinal fluid from the blood, thereby protecting the central nervous system from damage.

　　Principle of Experiment

　　The blood-brain barrier is composed of companion substances, the capillary wall of the choroidal flora and the glial membrane formed by the glial cells outside the wall. It is a natural barrier and is toxic to pathogenic microorganisms. You can stop the substance. Foreign objects including dye particles enter the brain tissue and cerebrospinal fluid from the blood, thereby protecting the central nervous system from damage.

　　Main reagent

　　1.5% triptan blue aqueous solution

　　2, 0.1ml of saline

　　Major equipment

　　1.1ml sterile syringe (with 4th needle)

　　2. Experimental materials, eye scissors, tweezers, etc.

　　2 mice

　　Experimental steps

　　1.1 Use a 1 ml sterile syringe to draw 5% triptan blue aqueous solution, draw 0.7 ml into each mouse from the tail vein, change the syringe, and then give it to one of the rats. 0.1ml saline was injected intracranially.

　　After 2.5-10 minutes, observe the color changes of the mouse skin, especially the eyes and mouth.

　　3. Within 30 to 60 minutes, the mouse's eyes and mouth looked blue, suffocated, and the abdomen was fixed downward.

　　4. Cut the skin along the dorsal midline from head to tail to expose the subcutaneous, muscles and internal organs, and observe the color change.

　　5. Carefully dissect the skull and vertebrae, expose the brain and spinal cord, compare subcutaneously, muscles and internal organs, and compare the differences between the two mice.

　　pay attention

　　1. Before use, the Tripan Blue solution must be filtered.

　　2. Tail vein injection must be inserted remotely. If the bolus is simple, it means it has entered the blood vessel. If it causes swelling or whitening under the skin, it means it is not in the blood vessels. Pull out the needle, and then insert the needle a little closer.