遗传编码纳米颗粒 z-bo 2020-10-19 352

　　The tools for sequentially regulating gene expression and cell function to clarify the process of physiological activity are invaluable and even have specific clinical therapeutic uses. Recent research papers have reported on a new type of genetic coding system that can be remotely controlled by low-frequency radio waves or magnetic fields. First, the heavy and light chains of ferritin labeled with green fluorescent protein are fused to form ferritin nanoparticles containing iron oxide as the intracellular core. Then, ferritin nanoparticles can interact with the anti-green fluorescent protein transient receptor potential vanillic acid 1 to form a fusion protein (aGFP-TRPV1).

　　Existing knowledge shows that transient receptor potential vanillic acid 1 (TRPV1) is a temperature-sensitive ion channel that can be opened when the temperature rises. Therefore, when the aforementioned fusion protein is formed, the iron oxide contained therein receives non-invasive radio or magnetic field signals and starts to generate heat, thereby activating ion channels. Through cell and mouse experiments, the researchers proved that TRPV1 has the ability to convert these force field signals with the help of iron oxide. The opening of ion channels leads to calcium-dependent gene transcription activation. Using a stem cell or viral expression system, the genetic component encoding the insulin gene is transferred to the mouse, and a radio or magnetic field is used to send a signal to successfully initiate the transcriptional expression of the insulin gene and respond accordingly. Reduce the blood sugar level of mice.

　　Finally, this powerful and reproducible remote control method in vivo can be used in basic scientific research, engineering research and clinical treatment to improve human health.