肠易激综合征内脏高敏模型 z-bo 2020-10-22 341

　　Objective To study different methods to make IBS visceral hypersensitivity immature rat models, and evaluate its visceral sensitivity and stability.

　　Method The SD rat pups on the second day after birth were randomly divided into a mother-infant separation group (group A), an unpredictable mother-infant separation group (group B) and a blank control group (group C). After successful modeling, observe the rat's general condition and stool condition, and determine the rat's visceral hypersensitivity and persistence by abdominal wall withdrawal reflex (AWR) score and visceral pain threshold. After the test, the rat colon tissue Perform pathological testing.

　　Results There were no significant differences in body weight and colonic histopathology between the groups (P>0.05); compared with group A and group C, the number of stools in group B increased and the water content of stool increased; compared with group C, group A and group C The AWR scores of rats in group B were higher and reached a peak at 9 weeks of age, while at 11 weeks of age, the AWR scores of rats in group A and B were lower than before; during the period of 5 to 9 weeks, compared with group C , The threshold of visceral pain caused by group A and group B was lower.

　　Conclusion Both mother-infant separation and unpredictable mother-infant separation can simulate the IBS visceral hypersensitivity model, and the unpredictable mother-infant separation model is more suitable for making diarrhea-type IBS models. At the same time, IBS visceral hypersensitivity is time-dependent, and the stable period of the model is mostly from the end of the model to the rat within 9 weeks of age.