小鼠帕金森模型 z-bo 2020-10-22 345

　　Objective To establish a stable 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced Parkinsonian model of C57BL/6 mice, and conduct behavioral evaluation of the model.

　　Methods Establish the administration group, the administration doses were 35mg/kg, 30mg/kg, 25mg/kg and the control group given 30mg/kg normal saline. Carry out tremor paralysis score, open field test, pole climbing test, suspension test, swimming test, and comprehensive statistics. The differences between the administration group and the control group and between the administration groups were analyzed. At the same time, HE and immunohistochemical TH antibody staining were performed to observe the changes of neurons and nerve fibers in the substantia nigra and striatum.

　　Results Compared with the control group, the scores of the administration group were significantly higher, but the behavioral scores of the 25 mg/kg dose group were significantly lower than that of the 35 mg/kg and 30 mg/kg dose groups. There was no significant difference between the 35 mg/kg and 30 mg/kg dose groups. difference. The number of TH-positive neurons in the substantia nigra area decreased, and the number of TH-positive nerve fibers in the striatum decreased.

　　Conclusion A simple way to evaluate the effect of mouse Parkinson's disease model through behavioral data has been established.