小鼠肺纤维化模型,博来霉素,内毒素 z-bo 2020-10-22 353

　　Objective To compare the degree of pulmonary fibrosis induced by bleomycin and endotoxin in two strains of ICR and C57BL/6 mice to establish a suitable pulmonary fibrosis model in mice.

　　Methods Two strains of ICR and C57BL/6 mice were randomly divided into control group, bleomycin group, and endotoxin group. The bleomycin group was injected with bleomycin (5 mg/kg body mass) into the trachea, the endotoxin group was injected with endotoxin (40 EU/kg body mass) into the tail vein, and the control group was injected with the same amount of normal saline. In the control group, bleomycin group, and endotoxin group of the two strains, 8 died at 7th, 14th, and 28th days after modeling. The general condition of the mice was observed and the lung coefficient of the mice was measured; the lungs were observed by HE staining Histopathological changes; ELISA method to detect the content of hydroxyproline in lung tissue.

　　Results Compared with the control group, the lung coefficient and hydroxyproline content of lung tissue of the two strains of the bleomycin group and endotoxin group increased to varying degrees, with significant differences (P<0.05). Among them, for the same pulmonary fibrosis inducer, the lung coefficient and hydroxyproline content of lung tissue of C57BL/6 mice were significantly different from those of ICR mice (P<0.05). for="" the="" same="" strain="" of="" there="" was="" no="" significant="" difference="" in="" lung="" hydroxyproline="" content="" tissue="" and="" endotoxin="" group="" bleomycin="" at="" different="" time="" points="" p="">0.05).

　　Conclusion C57BL/6 mice are more sensitive to bleomycin and endotoxin. Both bleomycin and endotoxin can prepare mice with nodular lung injury and pulmonary fibrosis.