SERS液滴 z-bo 2020-10-23 525

　　Recently, Dapeng Chen's group, a researcher at the Integrated Circuit Leading Process Research and Development Center of the Institute of Microelectronics, Chinese Academy of Sciences, and Professor Xiong Jijun's group from North University of China have made phased progress in surface-enhanced Raman (SERS) biochemical detection research.

　　Chen Dapeng's research group proposed an open SERS droplet sensor, which solves the complex preparation process problems required by traditional substrate-based SERS devices. It uses the porous fragility of the candle ash nano-chain structure to form a rich three-dimensional on the substrate in a rolling manner. "Hot spot" SERS active droplets, thereby enhancing the Raman detection performance of droplets. The liquid phase environment provided by the droplets can maintain the activity of biomolecules and improve the effective combination of biological macromolecules (such as proteins and DNA) with "hot spots". Therefore, the design and development of the SERS droplet sensor provides a new detection concept for liquid biopsy analysis and other technologies.

　　Compared with the sensors detected in the same liquid phase environment, this type of sensor shows higher detection ability, can detect (100fM) rhodamine 6G at a low limit, and can detect the functional probe molecules commonly used in liquid biopsy analysis (p-aminosulfide). Phenol and 2-mercaptopyridine) have strong detection specificity. The researchers verified the biodetection ability of the sensor by detecting bovine serum albumin (BSA), and the detection limit of BSA is as low as 1pM. The research shows that the sensor has application prospects for biomolecule detection.